Featuring PeakForce Tapping for Quantitative Bio-Mechanical Property Mapping

 

SAN Diego, California – December 15, 2014 – At the Sixth AFM BioMed Conference, Bruker (NASDAQ: BRKR) today announced the release of BioScope Resolve™, a biological atomic force microscope (bioAFM) that features the highest resolution imaging and most complete cell mechanics capabilities available for use with an inverted optical microscope (IOM). The system incorporates Bruker’s proprietary PeakForce Tapping® technology to enable researchers to achieve both the highest resolution imaging and piconewton level force measurements on biological samples. BioScope Resolve also provides real-time correlation of atomic force microscopy and optical microscopy data sets to provide unique insights into life sciences research. Ultimately, BioScope Resolve enables imaging and mechanical property mapping of the biological detail of cells, tissues, and biomolecular structures never previously observed with a bioAFM on an IOM.

“For the first time bioAFM analysis can directly correlate high-resolution molecular structures with function on the same cell,” said Dr. Lewis Francis of the College of Medicine and Center of NanoHealth at Swansea University, United Kingdom. “BioScope Resolveprovides the dynamic range and resolution necessary to allow a deeper understanding of structure-function relationships at cell and tissue surfaces.”

BioScope Resolve establishes a new benchmark for high-resolution imaging and cell mechanics characterization capabilities that are fully integrated with optical microscopy,” added David V. Rossi, Executive Vice President and General Manager of Bruker’s AFM business. “It builds upon our exclusive and leading PeakForce Tapping technology to enable the highest performance bioAFM that researchers of every experience level will find very easy to use.”

“PeakForce Tapping provided my lab with the force control and resolution necessary to produce ground-breaking ligand-receptor interaction maps using functionalized probes on live cells in a very time efficient and controlled way,” commented Professor Dr. Daniel Muller of the Department of Biosystems Science and Engineering at the ETH Zürich, Switzerland, about his experience using PeakForce Tapping.

About BioScope Resolve

BioScope Resolve is an AFM developed specifically for integration with optical microscopy for biological research into cells, tissues and molecules. The new system provides the most complete range of capabilities for cell mechanics and molecular force spectroscopy. Its design enables the highest resolution imaging for all types of biological samples, from cells to biomolecules, making it the new benchmark in high-resolution bioAFM capability. In addition, BioScope Resolve has complete synchronization with the top inverted optical microscope brands, and provides single-button imaging using proprietary ScanAsyst® technology. Researchers can utilize the system’s quantitative PeakForce QNM® nanomechanics package to map the surfaces of soft biological samples. BioScope Resolve’smechanical mapping capabilities bridge Force Volume and PeakForce QNM frequencies for the widest range available for force-distance curves on any AFM. BioScope Resolve also offers a full array of accessories to preserve the integrity of delicate samples, including carrier support with clear visual and physical access to samples, environmental control with heating and cooling capabilities, and a wide range of pre-calibrated biological probes.

About Bruker Corporation

Bruker Corporation is a leading provider of high-performance scientific instruments and solutions for molecular, cellular and materials research, as well as for industrial, diagnostics, clinical research and applied analysis.

Media Contact: 
Stephen Hopkins, Marketing Communications
Bruker Nano Surfaces Division
3400 East Britannia Drive, Suite 150, Tucson, AZ 85706
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker-nano.com

Investor Contact: 
Joshua Young
Vice President, Investor Relations
T: +1 (978) 663-3660 x1479
E: Joshua.young@bruker.com

World-Leading Graphene Research Facility Purchases Multiple Bruker AFMs

 

BILLERICA, Mass. – April 6, 2015 – Bruker Corporation (NASDAQ: BRKR) today announced an official partnership with the University of Manchester’s National Graphene Institute (NGI), joining a select list of industrial collaborators. This partnership follows NGI’s purchase of two additional atomic force microscopes (AFMs) from Bruker, a Dimension FastScan® and a Dimension Icon®. These systems join five other Bruker AFMs at the facility for research into the nanofabrication and nanoscale properties of graphene. As part of this partnership, Bruker will partially sponsor a Ph.D. student working on novel scanning probe microscopy (SPM) techniques to characterize graphene and 2D materials.

 

Graphene, the world’s thinnest, strongest and most conductive material, was first isolated and characterized at The University of Manchester by Sir Andre Geim and Sir Kostya Novoselov, who were awarded the Nobel Prize for Physics in 2010 for their research. This transparent, one-atom thick flat sheet of carbon has the potential to revolutionize technology, from smartphones and ultrafast broadband to drug delivery and computer chips. AFMs have demonstrated spatial resolution of less than a nanometer, and enable scientists developing materials such as graphene to understand critical mechanical, electrical, and chemical properties at the atomic scale.

 

“Our Bruker AFMs are anticipated to provide important new insights into nanoscale variations of graphene conductivity and work function,” said Nobel Prize winner Professor Novoselov. “Coupled with simultaneous quantitative mapping of mechanical properties, enabled by Bruker’s exclusive PeakForce Tapping®, NGI researchers hope to uncover new information that will ultimately optimize the performance of graphene-based materials and devices.”

 

“We are pleased that the National Graphene Institute continues to rely on our proprietary technology for its new world-class research facility, bringing their total now to seven Bruker systems,” added Paul Scagnetti, Ph.D., President of Bruker’s Nano Surfaces Division. He continued: “We are also gratified to be a part of the UK-wide Centre for Doctoral Training, where our unique AFM technologies will enable the sponsored Ph.D. student to learn even more about the physical and electrical properties of 2D materials under Professor Novoselov.”

 

About the UK National Graphene Institute 
The National Graphene Institute at The University of Manchester, UK, opened in March 2015, creating around 100 new jobs in a new, 7,800 square meter facility that will house state-of-the-art facilities, including two cleanrooms. The Institute will also feature a 1,500 square meter research lab for University of Manchester graphene scientists to collaborate with their colleagues from industry and other universities.

 

About Bruker Corporation 
For more than 50 years, Bruker has enabled scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Bruker’s high-performance scientific research instruments and high-value analytical solutions enable scientists to explore life and materials at molecular, cellular and microscopic levels.

 

In close cooperation with our customers, Bruker is enabling innovation, productivity and customer success in life science molecular research, in applied and pharma applications, in microscopy, nano-analysis and industrial applications, as well as in cell biology, preclinical imaging, clinical research, microbiology and molecular diagnostics.

 

Media Contact: 
Stephen Hopkins, Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker-nano.com

 

Investor Contact: 
Joshua Young
Vice President, Investor Relations
T: +1 (978) 663-3660 x1479
E: Joshua.young@bruker.com

SANTA BARBARA, California, September 30, 2014 – In a just released article in the Journal of Molecular Recognition, Dr. Hermann Schillers et al. report the first visualization of individual microvilli on living cells with atomic force microscopy. Microvilli are soft, microscopic cellular membrane protrusions present on epithelial cells that act to increase available surface area, enhance transmembrane transport, and serve as mechanosensors. Changes in their density and morphology are associated with diseases, such as celiac disease. As noted by Dr. Schillers, direct observation of microvilli on live cells had long eluded the AFM community.

“It was previously impossible to resolve the finest structures of a live cell like microvilli, but now with the BioScope Resolve, I can image them easily in one hour,” noted Dr. Schillers, lead author of the article. “This opens up exciting opportunities for new studies. Observing the structural and therefore functional integrity of microvilli on living cells will help to understand the development of microvilli-dependent diseases.”

Conventional AFMs subject live cells to high normal and lateral forces, yielding images that are dominated by the structure of the harder, underlying cytoskeleton and thus limiting their ability to track any changes in situ on the membrane of live cells. Bruker’s BioScope Resolve™ overcomes this challenge by probing the cell membrane at lower forces and with less spatial averaging from the extended probe structure and its movement through the viscous medium, bringing soft cell membrane structures into clear view for the first time.

About BioScope Resolve BioScope 
Resolve is an AFM designed specifically for the highest resolution imaging of all biological samples while on the inverted optical microscope. BioScope Resolve is the only AFM to resolve individual microvilli on live cells. It provides the most complete range of capabilities for cell mechanics and molecular force spectroscopy. Its exclusive PeakForce QNM® and FastForce Volume™ techniques deliver the highest resolution and fastest force mapping capabilities possible with an AFM and the widest range of force distance ramp rates. In addition, BioScope Resolve offers complete synchronization of AFM imaging and force spectroscopy with optical microscopy techniques, enabling new kinds of correlated experiments. BioScope Resolve is available with a full array of accessories including temperature and environmental control for live-cell imaging. For more information about Bruker’s BioAFM systems, please visit www.bruker.com/bioafm.

Media Contact: 
Stephen Hopkins, Marketing Communications
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

SANTA BARBARA, California – January 12, 2016 – Bruker’s Nano Surfaces Division today announced the release of the MultiMode 8-HR Atomic Force Microscope (AFM), which brings extensive new capabilities for nanomechanics and higher speed imaging to the world’s highest resolution, most widely-used and field-proven scanning probe microscope (SPM). The new nanomechanics features of MultiMode 8-HR enable researchers to access the broadest range of ramp frequencies for viscoelastic studies and nanomechanical assessment of a wide range of materials, from soft biological specimens to hard metallic samples.

“The MultiMode® platform has long been the gold standard for performance and application versatility, and we’ve significantly enhanced its capabilities with the MultiMode 8-HR,” commented Stephen Minne, Ph.D., Vice President and General Manager of Bruker’s AFM Business. “Unique improvements include higher speeds, higher resolution imaging, new capabilities in nanomechanics with enhanced PeakForce QNM®, and new FastForce Volume™ modes. In keeping with our ongoing commitment to our thousands of loyal MultiMode customers, we have designed these new developments in resolution, flexibility, and reliability to be available also as upgrades to their current MultiMode systems.”

About MultiMode 8-HR 
The high-resolution and data processing capabilities of the MultiMode 8-HR are the result of its combination of rigid, mechanical design and extremely advanced control electronics. Utilizing Bruker’s NanoScope® V Controller and new Version 8.2 software, the system features unprecedented bandwidth and extremely low-noise data acquisition to enable such proprietary technology advances as ScanAsyst®, Peak Force QNM and FastForce Volume. These features combine to reaffirm the MultiMode 8-HR as the most versatile, highest performance AFM in its class.

About Bruker Corporation
For more than 50 years, Bruker has enabled scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Bruker’s high-performance scientific research instruments and high-value analytical solutions enable scientists to explore life and materials at molecular, cellular and microscopic levels. In close cooperation with our customers, Bruker is enabling innovation, productivity and customer success in life science molecular research, in applied and pharma applications, in microscopy, nano-analysis and industrial applications, as well as in cell biology, preclinical imaging, clinical research, microbiology and molecular diagnostics.

Media Contact: 
Stephen Hopkins, Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

PORTO, Portugal – April 11, 2016 – At the 7th AFM BioMed Conference, Bruker’s Nano Surfaces Division today announced the release of RampScript™, which provides extensive new scripting capabilities for the BioScope Resolve® BioAFM. BioScope Resolve has set new standards for highest resolution AFM imaging with the first ever images of microvilli on live cells and by routinely resolving submolecular structures, such as the major and minor groove of DNA, while operating on an inverted microscope. With its combination of Bruker’s exclusive PeakForce Tapping® and FASTForce Volume™ technologies, BioScope Resolve already provides the widest range of ramp rates for biomechanics. Adding the most flexible ramp scripting interface available opens the door to new studies probing the dynamics of individual biomolecular bonds and the viscoelasticity of live cells.

“We are excited about the advances in cell mechanobiology that will be enabled by the new ramp scripting capabilities for BioScope Resolve,” said Marco Tortonese, Ph.D., Vice President and General Manager of Bruker’s AFM Instrumentation Business. “Atomic force microscopy can play an even larger role in cell mechanics research when a system has the capability to correlate with the most accurate data to fluorescence. This is why we made it such a priority to add the most comprehensive biomechanics functionality to a system specifically designed for highest resolution on an inverted optical microscope.”

About RampScript 
The RampScript package for BioScope Resolve delivers the most powerful mechanical investigation toolset available today. It complements the system’s widest range of ramp rates and allows users to build, control, and record complex nanomechanical measurements in such experiments as protein pulling, ligand-receptor interaction, and cell mechanics. RampScript features user-definable scripts for custom point measurements with step-by-step ramp definitions and simple drag-and-drop functionality for the ultimate experiment control. Ramps include seamless switching from segment to segment between open- and closed-loop operation, ramping and holding, Z-feedback and force feedback, as well as easy addition of TTL signals to synchronize optical microscopy or other external measurements. Bruker’s RampScript also enables dynamic mechanical analysis with frequency sweeps during ramp and hold measurements, at single points as well as integrated into force volume maps. A specially designed low-force trigger capability, together with fast, latency-free implementation build on the core performance of the BioScope Resolve to provide the most accurate script execution with pN force control.

Media Contact:
Stephen Hopkins, Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

Providing Nanometer-Resolution at High Scan Rates for up to 300-mm Samples

 

Santa Barbara, California – April 25, 2016 – Bruker today announced the release of the Dimension FastScan Pro™ Automated AFM Nano-Metrology System for high-volume measurement environments. FastScan Pro renders performance previously achievable only by AFM experts in research laboratories while delivering the repeatability and reproducibility expected in a production environment. The system integrates Bruker’s proven lowest noise and fast scanning technology to provide the highest throughput without reducing data quality. FastScan Pro also utilizes a highly comprehensive software automation package that features intuitive recipe-writing capabilities to reduce complex measurement routines to simple, push-button operations. This unique industrial AFM solution is completed with Bruker FastScan probes specifically designed to deliver longer tip life and sustained high-resolution data acquisition across hundreds of samples in high-volume environments. With its open access platform, large- or multiple-sample holders, and numerous ease-of-use features, FastScan Pro opens up the power of high-performance, research-grade atomic force microscopy to meet production requirements.

“Bruker has put considerable resources behind moving our unique, highly sophisticated research technologies into production applications,” said Marco Tortonese, Ph.D., Vice President and General Manager of Bruker’s AFM Instrumentation Business. “The FastScan Pro system’s industry-leading ease-of-use, highest resolution performance, greater than ten times measurement throughput increase, and capability to produce over five-hundred data images from a single probe should change the landscape of nanoscale quality control in manufacturing.”

About Dimension FastScan Pro

FastScan Pro incorporates both FastScan and Icon AFM scanners and Bruker-exclusive PeakForce Tapping® technology to perform accurate depth measurements from sub-nanometer steps to high-aspect ratio trenches with high accuracy. Robust software and an intuitive user interface support automated laser and detector alignment. Included is a built-in user-accessible cantilever database for system auto-settings, safe fast-engage control, and easy sample navigation. The system also features new AutoMET software, which delivers fast, automated metrology, exceptional ease-of-use, and AFM adaptability to capture critical-to-quality measurements needed in QA/QC, FA and production.

Media Contact: 
Stephen Hopkins
Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

PeakForce sMIM Mode Provides Enhanced Nanoscale Mapping of Permittivity and Conductivity

 

SANTA BARBARA, California – May 17, 2016 – Bruker’s Nano Surfaces Division today announced the release of scanning microwave impedance microscopy (sMIM) capability for its Dimension Icon® atomic force microscope (AFM) platform. The innovative sMIM technique works by reflecting a microwave signal from a nano-probe and sample interface to reveal the electrodynamic properties of the sample surface and sub-surface. The detection and processing of microwave reflectance is done in real time, allowing direct access to the permittivity and conductivity of materials. When integrated with Bruker’s unique PeakForce Tapping®, sMIM greatly expands its applications to simultaneous characterization of electrical and mechanical properties. In addition, Bruker’s new PeakForce sMIM mode enables characterization of previously challenging measurements on fragile samples, such as nanotubes, nanowires, biological samples and is ideal for electrical characterization of 2D materials.

“We greatly expanded the nano-electrical characterization capabilities for researchers in the semiconductor industries by bringing Bruker’s exclusive PeakForce Tapping to the exciting sMIM technology developed by PrimeNano Inc.” said Marco Tortonese, Ph.D., Vice President and General Manager of Bruker’s AFM Instrumentation Business. “Researchers can now benefit from the most sensitive and complete permittivity and conductivity data on even the most delicate of materials, all at the nanoscale.”

“Our sMIM technique is already changing the way nanoelectric research is being done, and we are gratified that the electronics and proprietary probes we have developed are benefitting the entire AFM community,” added Dr. Stuart Friedman, CEO of PrimeNano, Inc. “PrimeNano’s enabling sMIM technology being utilized with Bruker’s well-known Icon system, particularly in conjunction with their PeakForce Tapping mode, is another huge step in this endeavor.”

About PeakForce sMIM
The AFM-based sMIM technique accesses the reflected microwave signal from the tip-sample interface to reveal electrodynamic property data of the sample surface underneath the tip. Since it is based on the capacitive coupling between the tip and the sample, sMIM does not require making electrical contact between the sample and the substrate. This frees researchers and engineers from the tedious work of wiring and soldering that might alter the sample electrical properties and be impossible for nanoscale materials. As a near-field method, the resolution of sMIM is only limited by the tip radius of the probe, and it can easily achieve a lateral resolution of <20 nm for electrical mapping. Sub-aF sensitivity and high S/N ratios are realized by using waveguide tips with coaxial shielding. Having these unique capabilities, sMIM is superior to other AFM-based electrical modes for a broad range of applications. The versatility of PeakForce sMIM leveraging Dimension Icon and PeakForce Tapping will empower material researchers and device engineers to explore basic principles underlying functionality and perform more advanced and complete materials characterization and device failure analysis.

Media Contact: 
Stephen Hopkins,
Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

SANTA BARBARA, CA – September 20, 2016 – Bruker’s Nano Surfaces Division today announced the release of scanning electrochemical microscopy (SECM) capability for its Dimension Icon® atomic force microscope (AFM) platform. Utilizing a proprietary probe design, Bruker’s new PeakForce SECM™ mode controls nanoelectrode tip position and tip-sample interaction with unprecedented precision to provide simultaneous capture of topographical, electrochemical, electrical, and mechanical maps. This capability provides access to previously unobtainable nanoscale observation of redox reactions and their kinetics. Now researchers are able to address vital evolution of materials for energy, environment, and biochemical sensors.

“Our customers have been consistently imaging at very high resolution with the new nanoelectrodes on the Dimension Icon AFM,” said Teddy Huang, Ph.D., Senior Applications Scientist at Bruker Nano Surfaces Division. “For the first time they are able to capture electrochemistry correlated with nanomechanical information to understand the complex relationship between size, geometry, modulus, adhesion, and activity of nanostructured materials.”

PeakForce SECM radically redefines what is possible in the nanoscale visualization of electrical and chemical processes in liquid,” added Marco Tortonese, Ph.D., Vice President and General Manager of Bruker’s AFM Instrumentation Business. “This breakthrough is the result of the continuing evolution of PeakForce Tapping® technology combined with our in-house design and manufacture of reliable commercial probes.”

About PeakForce SECM

PeakForce SECM takes full advantage of Bruker’s exclusive PeakForce Tapping to deliver a new approach to SECM that extends its capability to nanoscale electrochemistry, electrical and mechanical mapping of a wide variety of samples, from soft biological systems to polymers. Bruker’s PeakForce SECM probes are batch-fabricated using proprietary methods to consistently produce a high-quality nanoelectrode. An optimized conductive path and special coating techniques eliminate pinholes, resulting in negligible leakage and capacitive currents. PeakForce SECM improves resolving power over traditional approaches by orders of magnitude, and opens the door to electrochemical measurements on individual nanoparticles, nanophases, and nanopores.

About Bruker Corporation

For more than 50 years, Bruker has enabled scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Bruker’s high-performance, scientific research instruments and high-value analytical solutions enable scientists to explore life and materials at molecular, cellular and microscopic levels.

In close cooperation with our customers, Bruker is enabling innovation, productivity and customer success in life science molecular research, in applied and pharma applications, in microscopy, nano-analysis and industrial applications, as well as in cell biology, preclinical imaging, clinical research, microbiology and molecular diagnostics.

Media Contact:

Media Contact: 
Stephen Hopkins
Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com

Dimension FastScan Bio Provides Advantages over Conventional AFMs

 

SANTA BARBARA, California – November 1, 2016 – Bruker’s Nano Surfaces Division reports on the groundbreaking use of a Dimension FastScan Bio™ AFM system at the University of Leeds, UK, to investigate biological and biomolecular systems at the highest resolution. Neil Thomson’s group, associated with the University’s Astbury Centre for Structural Molecular Biology, has utilized the system’s high-speed scanning, exceptional force control and high-resolution performance to characterize DNA systems, such as DNA-protein interactions and DNA origami structures, in ways that were not previously possible with other instrumentation. Furthermore, Simon Connell’s team in the School of Physics and Astronomy, where the instrument is based, has characterized the temperature-dependent dynamic behavior of critical biomimetic lipid membranes.

“The FastScan Bio AFM has improved performance over conventional AFMs, as we have achieved resolution close to the helical repeat of the DNA strands that make up the origami nanotiles,” explained Professor Neil Thomson. “The smaller FastScan probes and high stability of this AFM platform are key to obtaining high-resolution imaging under liquid. This improved resolution is helping us answer questions about the formation and stability of DNA origami for future nanomaterials applications.”

“The higher speed and stability of the FastScan Bio gives us the first direct insight into membrane nano-domain dynamics,” added Dr. Simon Connell. “The movies we capture of motion in model cell membranes under increasing temperature let us quantify domain line tensions and observe the breakdown of large lipid domains into much smaller nanoscale fluctuations. We can now directly correlate domain lifetime with size at and above the critical temperature.”

“Bruker has spent considerable effort to bring the performance and speed benefits of our FastScan platform to life science applications,” explained Marco Tortonese, Ph.D., Vice President and General Manager of Bruker’s AFM Instrumentation Business. “The FastScan Bio’s combination of resolution and productivity enables more groups like those at the University of Leeds to observe biological functions at AFM spatial resolution, ultimately increasing the pace of biological discovery.”

About Dimension FastScan Bio

The Dimension FastScan Bio system utilizes a revolutionary XYZ scanner designed to operate at high-speed rates while delivering extremely low drift and low noise. Together with its large XYZ scan range, this enables high-resolution and high-speed imaging of both molecular and cellular dynamic processes. The system’s innovative user interface, including automated laser and detector alignment, feature-tracking capabilities with NanoTrackTM, and real-time data analysis with MovieMakerTM, not only reduces the complexity of the experimental setup, it also dramatically increases the success to acquiring biologically impactful data. Other accessories include a micro-volume flow cell for controlled fluid exchange, as well as a sample stage heater for conducting studies at physiologically relevant temperatures. With the FastScan Bio, productive temporal, high-resolution nanometer-scale research is a reality for every bio researcher.

Media Contact:

Media Contact: 
Stephen Hopkins
Marketing Communications
Bruker Nano Surfaces Division
T: +1 (520) 741-1044 x1022
E: steve.hopkins@bruker.com